BIOLOGY 103
FALL, 2002
LAB 5

Molecules and Chemical Reactions


Name:  
Username:  Anonymous
Subject:  Oxygen levels
Date:  2002-10-08 15:10:03
Message Id:  3205
Comments:
Brenda Zera, Elizabeth Damore

All measurements were taken at 15 second intervals.

First experiment: 0, .2, .4, .4, .6, 1, 1.2, 1.4, 1.6, 1.8, 2, 2, 2.2, 2.2, 2.4, 2.6, 2.6, 2.8, 2.8, 3, 3, 3.

After change in H2O2 level (Experiment #2): .4, .4, .5, .6, .7, .8, 1, 1.1, 1.3, 1.4, 1.5, 1.6, 1.7, 1.7, 1.8, 1.9, 2, 2, 2, 2.2, 2.2, 2.3, 2.4

We used less H2O2 in the second experiment which raised the rate of reaction, resulting in a lower production of oxygen within the same time period.

Reaction Rate (First experiment)= .4
Reaction rate (second experiment)= .7


Name:  amanda maclay, diana fernandez
Username:  Anonymous
Subject:  experiment 5
Date:  2002-10-08 15:15:48
Message Id:  3206
Comments:
135 seconds- .9
150 s.- 1.0
165s. 1.0
180 s.- 1.1
195 sec. - 1.1
210 sec. - 1.1
225 sec. - 1.1
240sec. - 1.1
255 sec. 1.2
270 sec. - 1.2
285 sec. - 1.2
300 sec. - 1.2

reaction rate = (1.2-.9)/3 = .1/1 minute

method: our portion of the experiment was to see if changing temperature would affect reaction time of the enzyme. We hypothesized that temperature woudl slow down the reaction time by making the oxygen release per minute lower than the test run that we first ran.
we found that the reaction time during trial one of the experiment was much higher (.8/1minute) than that of our reaction time for experiment number five.
mande adn debe


Name:  MaryBeth & Ginnie
Username:  vculler@brynmawr.edu
Subject:  Catalase Lab
Date:  2002-10-08 15:23:14
Message Id:  3207
Comments:
Experiment #1 and Experiment #3, MaryBeth Curtiss and Virginia Culler

Trial #1: Results for 15 second time intervals:
.2, .3, .4, .6, .6, .8, 1.0, 1.4, 1.8, 2.0, 2.4, 2.4, 2.6, 2.8, 2.9, 3.0, 3.2, 3.4, 3.5, 3.6, 3.5, 3.6, 3.6, 3.7, 3.8, 3.9, 4.0, 4.1, 4.1, 4.1
Total oxygen: 4.1cc
Trial#2: Results for 15 second intervals, more catalase:
.1, .5, .7, 1.0, 1.4, 1.7, 2.0, 2.2, 2.5, 2.6, 2.8, 3.0, 3.1, 3.2, 3.2, 3.3, 3.4, 3.5, 3.6, 3.6, 3.7, 3.8, 3.9, 3.9, 3.9
Total oxygen: 3.9cc
Trial #3: Results for 15 second intervals, same catalase as Trial#2:
.2, .5, .8, 1.3, 1.7, 2.0, 2.2, 2.4, 2.7, 2.8, 3.0, 3.2, 3.3, 3.3, 3.6, 3.6, 3.6
Total oxygen: 3.6

Rates of oxygen conversion for each trial:
Trial #1: .5857 cc of O2/minute*
Trial #2: .7667 cc of O2/minute
Trial #3: .7667 cc of O2/minute

*For the Trial #2 and #3 calcualtions, we used a span of three minutes to determine the rate of oxygen production because the actual total time elapsed was shorter than the first trial. To calculate the Trial #1 rate, we used the total span of seven minutes.

The results of the experiment were mostly what we expected to observe. The second and third trial were shorter and more productive because more of the catalase was added, and the rate results, though the specific numbers sometimes varied, were the same. The first trial took longer to produce the same amount of oxygen because the first vial had less of the catalase enzymes.

Name:  kyla &laura
Username:  kellis@brynmawr.edu
Subject:  
Date:  2002-10-08 15:23:54
Message Id:  3208
Comments:
Kyla Ellis
Laura Silvius
10/8/02
Volume Of Oxygen Produced Over Time By Degradation Of Hydrogen Peroxide By Catalase

Experiment 1, Trial 1 (Volume of Oxygen in CCs, taken at 15 second intervals):
0,0,0,.2,.4,.5,.62,1,1.1,1.2,1.4,1.5,1.6,1.8,2,2.2,2.3,2.4,2.6,2.6,2.7,2.8,2.9,3,3.1,3.2,3.3,3.4,3.5,3.6,3.6,3.7,3.7,3.8,3.9,3.9,3.95,3.95,4,4.1,4.2,4.2,4.2,4.2
Reaction Rate:.533

Experiment 2, Trial 1:
.4,.4,.5,.6,.8,1,1.2,1.4,1.8,2,2.2,2.4,2.6,2.9,3,3.2,3.3,3.6,3.7,3.9,4,4.1,4.4,4.5,4.6,4.7,4.8,4.9,4.9,5,5.1,5.18,5.2,5.2,5.3,5.35,5.4,5.5,5.5,5.6,5.7,5.8,5.8,5.8,5.8
Reaction Rate:.83

Experiment 2, Trial 2:
0,.1,.2,.5,.7,1,1.2,1.4,1.6,1.8,2.2,2.2,2.4,2.6,2.7,2.9,3.2,3.2,3.4,3.7,3.9,4.1,4.2,4.3,4.4,4.6,4.6,4.8,4.8,4.9,5,5,5.1,5.2,5.2,5.2,5.3,5.4,5.4,5.4
Reaction Rate: .76

When we increased the amount of H2O2, the reaction became quicker and more noticeable to the naked eye.
We conclude that as the ratio between the two components decreases, the reaction becomes more rapid and stronger.


Name:  the Ks
Username:  Anonymous
Subject:  Chemical Reactions
Date:  2002-10-08 15:25:54
Message Id:  3209
Comments:
This experiment was conducted by the Ks-
Katie Campbell
Kate Amlin

(All volume units are in cc.)

EXPERIMENT #1:Standard Catalase at Room Temp.

DATA: .1,.2,.3,.4,.6,1.0,1.2,1.5,1.5,1.8,2.0,2.3,2.4,2.5,2.7,2.8,
2.9,3.0,3.2,3.3,3.5,3.6,3.7,3.7,3.7

REACTION RATE: Volume at 2 minutes = 1.5cc
Volume at 4 minutes = 2.8cc
(2.8cc - 1.5cc)/2 = 0.65cc/min

EXPERIMENT #4:Change of pH of Buffer
(Due to time constraints we were only able to conduct one trial.)

DATA: 0.0,0.0,0.0,0.0,0.1,0.2,0.2,0.2,0.3,0.3,0.4,0.5,0.6,0.6,0.7,0.8,
0.8,0.9,1.0,1.1,1.2,1.2,1.3,1.4,1.4,1.5,1.6,1.7

REACTION RATE: Volume at 2 minutes = 0.2cc
Volume at 4 minutes = 0.8cc
(0.8cc - 0.2cc)/2 = 0.3cc/min

CONCLUSION: The pH effects the reaction rate. Lowering the pH
significantly slowed the rate of the reaction between the
enzyme and the hydrogen peroxide.

Obviously the enzyme reacts with the hydrogen peroxide to release the oxygen. The pH only effects the reaction time. It does not effect the amount of oxygen that is produced by the chemical reaction. As you can see from our second experiment in which we changed the pH of the buffer, the reaction would still produce the same amount of oxygen that the first experiment did but at a much slower pace.


Name:  
Username:  Anonymous
Subject:  Lab
Date:  2002-10-08 15:28:43
Message Id:  3211
Comments:
In our experiment, 2C, we increased the level of H2O2, or peroxide. The x was up, the rate was up. The length of time it took for the reaction to comlete itself was longer.


This is our data:
Experiment 1:
0, .2, .4, .6, .8, 1.0, 1.2, 1.4, 1.5, 1.6, 1.65, 1.8, 1.9, 2.0, 2.0, 2.05, 2.1, 2.15, 2.2, 2.2, 2.3, 2.35, 2.4, 2.4, 2.4, 2.4
Total time: 6 minutes 30 seconds
Rate: .2 ml/min

Experiment 2C:
Trial 1:
.2, .35, .6, .85, 1.2, 1.5, 1.8, 2.0, 2.3, 2.6, 2.8, 3.0, 3.2, 3.4, 3.6, 3.7, 3.8, 4.0, 4.1, 4.2, 4.25, 4.4, 4.5, 4.6, 4.6, 4.6, 4.65, 4.7, 4.8, 4.8, 4.8, 4.8
Total time: 8 minutes
Rate: .6 ml/min

Trail 2:
.1, .2, .4, .6, .8, 1.1, 1.4, 1.7, 2.0, 2.2, 2.4, 2.7, 3.0, 3.1, 3.3, 3.4, 3.6, 3.8, 4.0, 4.1, 4.3, 4.4, 4.5, 4.6, 4.65, 4.8, 4.8, 5.0, 5.0, 5.1, 5.2, 5.2, 5.4, 5.4, 5.4, 5.4
Total time: 9 minutes
Rate: .7 ml/min

Trial 3:
.1, .2, .4, .6, .8, 1.1, 1.4, 1.7, 2.0, 2.2, 2.4, 2.6, 2.7, 3.0, 3.1, 3.2, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4.0, 4.0, 4.2, 4.2, 4.3, 4.4, 4.4, 4.4, 4.5, 4.6, 4.6, 4.6, 4.7, 4.8, 4.8, 4.8, 4.8
Total time: 10 minutes
Rate: .52 ml/min

Christine Traversi
Heather Price


Name:  Jen + Stephanie
Username:  jrusk@brynmawr.edu
Subject:  oxygen levels
Date:  2002-10-08 15:31:56
Message Id:  3212
Comments:
Stephanie and Jen
Original Results
.2
.8
.9
1.0
1.2
1.4
1.6
1.8
2.0
2.2
2.4
2.6
2.8
2.8
3.0
3.2
3.4
3.4
3.6
3.8
3.8
3.8
3.9
4.0
Original Reaction rate: .070

Trial B Results
.6
.8
.8
1.0
1.2
1.3
1.5
1.8
2.0
2.2
2.4
2.6
2.8
3.0
3.2
3.3
3.4
3.5
3.6
3.8
3.9
4.0
4.1
4.2
Trial B reaction rate: .075
(Trial A reaction rate: .015)

Methods: Every fifteen seconds recorded reaction measurement from the injection of the H2O2.

Observations: We found a variety of observations. In trial A, nothing really moved and when it did, it was slow. In Trial B, the results were similiar to the original.

Our enzyme did not change because our rate stayed the same as X went up.


Name:  Kathryn Bailey, Sarah Frayne
Username:  Anonymous
Subject:  Results
Date:  2002-10-08 15:33:20
Message Id:  3213
Comments:
Trial 1
(Room Temp)

0,0,0,0,0,.1,.2,.2,.4,.4,.4,1.1,1.3,1.5,1.5,1.8,2.0,2.2,2.2,2.3,2.4,2.6,2.8,2.8,2.8

Reaction Rate: 0.7 O2/minute

Trial 2
(Increased Temp)

0,.1,.4,.6,1.0,1.3,1.6,1.8,2.2,2.4,2.8,3.0,-,-,-,3.8,3.8,4.0,4.1,4.2,4.2,4.3,4.3,4.3

Reaction Rate: .8 O2/minute

Trial 3
(Increased Temp)

0,0,.2,.6,.8,1.0,1.4,1.6,1.8,2.2,2.4,2.8,3.2,3.2,3.2,3.2,3.5,3.8,4.0,4.0,4.1,4.1,4.2,4.2,4.3,4.3,4.3

Reaction Rate: .8 O2/minute


Our data shows that the reaction rate was slightly faster when the chemicals at higher temperatures. The amount of overall O2 volume was higher at the larger temp, however we only did one trial at room temperature, toconfirm this change we would need to do another trial at room temp. Our story is that the hightened temperature allowed for a faster reaction rate because the molecules were moving around faster. that's our story and we're sticking to it....for a while anyway.....


Name:  Yarimee Gutierrez Joanna Rober
Username:  jjroberts@brynmawr.edu
Subject:  
Date:  2002-10-08 15:36:57
Message Id:  3214
Comments:
Yarimee Gutierrez
Joanna Robertson
Ex #1 Ex #2
15-0 15-0
30-.1 30-0
45-.2 45-.1
60-.3 60-.1
75-.4 75-.1
90-1.0 90-.1
105-.1.1 105-.1
120-1.2 120-.1
135-1.3 135-.1
150-1.4 150-.1
165-2.0 165-.2
180-2.1 180-.2
195-2.2 195-.2
210-2.2 210-.2
225-2.3 225-.3
240-2.3 240-.3
255-2.4 255-.3
270-2.4 270-.3
285-3.0 285-.3
300-3.0 300-.3
315-3.1 315-.3
330-3.1 330-.3
345-3.1 345-.3
360-3.1 360-.3

Rate of Oxygen produced:
Ex 1 (control):0.6 cc/min
Ex 2 (pH 2.0): 0.067 cc/min

Observations:
-Reaction rate significantly decreased.
-x(plateu value) significantly decreased.
Story- The pH level affects how the Catalase works.


Name:  Michele and Heidi
Username:  Anonymous
Subject:  
Date:  2002-10-09 14:15:27
Message Id:  3226
Comments:
Heidi Adler-Michaelson & Michele Doughty

Trial 1:
.2, .4, .8, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6, 2.8, 3.0, 3.0, 3.2, 3.3, 3.4, 3.4, 3.5, 3.6, 3.6, 3.7, 3.8, 3.8, 4.0, 4.0, 4.0
The rate of change is fairly constant for 4 minutes at an additional .2 cm for every 15 seconds. After 4 minutes it changes .2 cm for every 30 seconds until it stabilizes after 6 min 40 sec reaching 4.0 cm of oxygen in the tube.


Name:  
Username:  Anonymous
Subject:  Breakin' Down H2O2 Baby!!!
Date:  2002-10-09 14:47:25
Message Id:  3227
Comments:
Melissa Brown and Roma Hassan

Introduction:
This experiment demonstrates the effects of the enzyme Catalase on hydrogen peroxide.

Methods:
We were given a nicely set-up apparatus consisting of a ring stand and a Scholander respirometer. We had two vials one of which was a control vial and the other was the experimental vial. In the control vial we put in 0.5cc of H2O2 and 4.5cc of pH 7.4 buffer and in the experimental vial we first put in 1.5cc of Catalase and 3cc of the buffer. The two vials were attached to the respirometer and then 0.5cc of H2O2 was injected into the experimental vial and the small plug was pushed in and at the same time the oxygen syringe was slowly pulled up and different volumes of oxygen were measured for different time intervals. The methods are the same for both trial 1 and trial 2.

Observations:
Trial 1
Time/sec Volume O2
15 .2
30 1
45 1.6
60 2.2
75 2.7
90 3
105 3.1
120 3.2
135 3.2
150 3.4
165 3.4
180 3.6
195 3.8
210 3.8
225 3.9
240 3.9
255 3.9
270 3.9
. .
. .
. .
. .

Trial 2(performed twice)
Time/sec Volume O2
15 .2/.4
30 .9/.8
45 1.4/1.4
60 1.8/1.8
75 2.2/2.0
90 2.4/2.4
105 2.7/2.7
120 2.8/2.8
135 3.0/3.0
150 3.2/3.2
165 3.2/3.3
180 3.4/3.4
195 3.6/3.5
210 3.8/3.6
225 3.9/3.8
240 3.9/3.9
255 3.9/3.9
270 3.9/3.9
. .
. .
. .
. .

Conclusion/ThE StoRY:
Rate of Concentration= 1.6/2= 0.8cc of O2/min
Our experiment remained the same, thus observations remained similar and hence we conclude with our rate of concentration and alas, no remarks on enzyme behavior!!!


Name:  Brie Farley, Anastasia Michals
Username:  Anonymous
Subject:  Lab 5
Date:  2002-10-09 14:50:31
Message Id:  3228
Comments:
Our results from experiment one were:
DATA: .5, 1, 1.5, 2, 2.5, 2.9, 3, 3.5, 3.8, 4, 4, 4, 4.1, 4.1, 4.1, 4.1
Our data was collected over a period of four minutes. This experiment was the first trial of adding .50 H2O2 to the experimental mixture, which contained 1.50 ml of Catalase and 3 ml of 7.4 Buffer for a total volume of 5.00 ml.

Our results from experiment two were:
DATA: .1, .5, 1, 1.5, 1.9, 2, 2.2, 2.4, 2.4, 2.6, 2.6, 2.6
Our data was collected over a period of three minutes. This experiment was the first trial of adding .40 H2O2 to the experimental mixture, which contained 1.50 ml of Catalase and 3.10 ml of 7.4 Buffer for a total volume of 5.00 ml.

Our results from the second trial of the same experiment were:
DATA: .1, .5, 1, 1.4, 1.8, 2.1, 2.2, 2.4, 2.6, 2.6, 2.8, 2.8

In the first experiment the rate we computed over two minutes was 1 O2/minute. In the second experiment the rate we computed over two minutes was .9 O2/min. When we add less H2O2, we ended up with less O2 per minute, but our rate was the same in both experiments. This means that the rate is independent of the amount of H2O2 that is added. Our conclusion is that the rate is determined by the enzyme that is added. Since the amount of enzyme was the same in both experiments, the rate should stay the same. Since it did, our conclusion has been proven.


Name:  Maggie Scott-Weathers and Emil
Username:  esenerth and mscottwe
Subject:  
Date:  2002-10-09 14:59:56
Message Id:  3229
Comments:
Experiment 1:

1.8, 2.1, 2.4, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.25, 3.3, 3.3, 3.35, 3.4, 3.45, 3.5, 3.55, 3.6, 3.6, 3.6

Experiment 4B:
(Experiment 4 was using a buffer of 4.01 pH instead of the previously used pH level of 7.4)
Trial 1: .2 cc after 15 seconds
Trial 2: .2 cc after 15 seconds.

In both of our trials for experiment 4, the brody shot up quickly, equalling .2 cc, but then stopped moving completely.

This made us come to the conclusion that the higher the buffer's pH is, the longer the reaction will run, and the lower the buffer's pH is, the shorter the time of the reaction. This may mean that the lower the buffer's pH, the less effective the catalase (the enzyme) is at breaking apart the peroxide.

Our rate = .2/15= .01333 cc/sec. However, this rate could be deceiving because while we took the first measurement at fifteen seconds, the oxygen had stopped moving before that.


Name:  Annie S., Catherine R.
Username:  Anonymous
Subject:  October 9th Lab
Date:  2002-10-09 15:05:32
Message Id:  3230
Comments:
Trial 1

Time (seconds) / Volume of 02
15 / -
30 / -
45 / 2.6
60 / 3.0
75 / 3.6
90 / 3.8
105 / 4.0
120 / 4.2
135 / 4.4
150 / 4.6
165 / 4.7
180 / 4.8
195 / 4.9
210 / 4.9
225 / 5.0
240 / 5.0
255 / 5.0
270 / 5.0
285 / 5.1
300 / 5.1
315 / 5.1
330 / 5.1
345 / 5.1
360 / 5.1

Trial 2

Time (seconds) / Volume of 02
15 / 0.8
30 / 2.1
45 / 2.4
60 / 2.8
75 / 3.0
90 / 3.1
105 / 3.2
120 / 3.3
135 / 3.4
150 / 3.5
165 / 3.6
180 / 3.6
195 / 3.7
210 / 3.75
225 / 3.8
240 / 3.8
255 / 3.85
270 / 3.9
285 / 3.9
300 / 3.95
315 / 4
330 / 4
345 / 4
360 / 4

Trial 3

Time (seconds) / Volume of 02
15 / 1.8
30 / 2.2
45 / 2.3
60 / 2.6
75 / 2.8
90 / 3.0
105 / 3.2
120 / 3.25
135 / 3.3
150 / 3.4
165 / 3.4
180 / 3.5
195 / 3.6
210 / 3.6
225 / 3.65
240 / 3.7
255 / 3.75
270 / 3.8
285 / 3.8
300 / 3.85
315 / 3.85
330 / 3.85
345 / 3.85
360 / 3.9

-We decreased the amount of H2O2 in the system, predicting that this decrease would produce less oxygen.
-Our data proves there is a direct corrollation between the two variables.
-Decrease in the amount H2O2 lowers the rate of oxygen production in relation to time from about 0.9 to approximately 0.425.
Name:  Heidi & Michele
Username:  Anonymous
Subject:  
Date:  2002-10-09 15:07:01
Message Id:  3231
Comments:
Heidi Adler-Michaelson & Michele Doughty

Trial A: (at room temperature)
.2, .4, .8, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.6, 2.8, 3.0, 3.0, 3.2, 3.3, 3.4, 3.4, 3.5, 3.6, 3.6, 3.7, 3.8, 3.8, 4.0, 4.0, 4.0
The rate of change was fairly constant for 4 minutes at an additional .2 cm for every 15 seconds. After 4 minutes it changed .2 cm for every 30 seconds until it stabilized after 6 min 40 sec reaching 4.0 cm of oxygen in the tube.

Our rate between 1 and 3 minutes was 0.80 O2 per minute.

Trial B: (at heated temperature)
test #1
.3, .8, 1.3, 2.0, 2.4, 2.7, 3.0, 3.2, 3.4, 3.6, 3.7, 3.8, 4.0, 4.1, 4.2, 4.2, 4.3, 4.4, 4.4, 4.5, 4.6, 4.6, 4.6, 4.7, 4.8, 4.8, 4.8, 4.9.

Our rate between 1 and 3 minutes was 0.90 O2 per minute.

test#2
.2, .8, 1.4, 2.0, 2.5, 2.8, 3.2, 3.4, 3.6, 3.8, 4.0, 4.1, 4.2, 4.4, 4.4 4.6, 4.6, 4.7, 4.8, 4.8, 4.9, 5.0, 5.0, 5.0

Our rate between 1 and 3 minutes was 1.05 O2 per minute.

** Our average rate of O2 per minute for Trial B was 0.98***

Discussion:
With higher temperature the rate of the O2 per minute was greater than the rate of the room temperature by a difference in rate of 0.18 O2 per minute.


Name:  MC the amaaaazing
Username:  clphilli@brynmawr.edu
Subject:  We love Hiooouuueeee
Date:  2002-10-09 15:07:58
Message Id:  3232
Comments:
Mer, Chels(ea)

The control trial involved 1.5cc of the Catalase, 0.50cc of the Hydrogen Peroxide, and 3.00cc of the buffer. The readings were taken every 15 seconds.

Our control set of data was as follows:

.4, .8, 1.2, 1.6, 2, 2.2, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.1, ~3.2, 3.2, 3.2,

3.3, 3.3, 3.4, 3.4, 3.4


Our next trials involved 0.6cc of Hydrogen Peroxide, 2.9cc of the buffer, and the enzyme remained constant at 1.5cc. The rate from our first trial (for minutes 1-3) was 0.7cc/minute of oxygen; the rate from the data set below with the increased amount of Hydrogen Peroxide is 1cc/ minute. Our data for the second set was as follows:

.3, 1.0, 1.8, 2.4, 2.8, 3.2, 3.5, 3.7, 3.9, 4.1, 4.2, 4.4, 4.4, 4.5, 4.6,

4.7, 4.8, 4.8, 4.9, 5.0, 5.0, 5.1, 5.2, 5.2, 5.2

Based on our scientific observations, we have concluded that increasing the amount of HP added to the enzyme increases the rate at which oxygen converts to a gas. NOTE: Although the time is different on each of these trials, we feel that it is not really legitimate to say that the amount of HP affects time as well because our first trial was shorter than the control.


Name:  Chelsea and Carrie
Username:  cgriffin@brynmawr.edu
Subject:  Hydrogen Peroxide and Catalase Lab
Date:  2002-10-09 15:09:28
Message Id:  3233
Comments:
Trial 1:
Time (in seconds) -- Volume of Oxygen (in mL)
15 -- .2
30 -- .6
45 -- 1.2
60 -- 1.8
75 -- 2
90 -- 2.4
105 -- 2.6
120 -- 2.8
135 -- 3.0
150 -- 3.2
165 -- 3.2
180 -- 3.4
195 -- 3.6
210 -- 3.6
225 -- 3.8
240 -- 3.8
255 -- 3.9
270 -- 4.0
285 -- 4.0
300 -- 4.0
Rate of Reaction: .8 mL of Oxygen/minute

Summary of Data from Trial 2 (with same substances, but chilled):
Volume of Oxygen: .2, .2, .6, .8, 1, 1.2, 1.6, 1.8, 2, 2, 2.2, 2.4, 2.4, 2.6, 2.6, 2.8, 2.8, 3.0, 3.0, 3.0, 3.2, 3.2, 3.2, 3.3, 3.3, 3.4, 3.4, 3.4, 3.5, 3.6, 3.6, 3.6, 3.7, 3.7, 3.8, 3.8, 3.8, 3.8, 3.8,3.8, 3.8
Rate of Reaction: .3 mL of Oxygen/minute

Summary of Data from Trial 3 (with same substances, but chilled):
Volume of Oxygen:.2, .4,.8, 1. 1.4, 1.7, 2, 2.2, 2.4, 2.6, 2.8, 3.0, 3.0, 3.2, 3.4, 3.6, 3.6, 3.7, 3.8, 3.8, 3.9, 4.0, 4.0, 4.0, 4.1, 4.1, 4.1, 4.1, 4.2, 4.2, 4.2, 4.2
Rate of Reaction: .3 mL of Oxygen/minute

Cooler temperatures mean slower molecules. Thus, when all the solutions were chilled, their slower molecular movement accounts for the drop in reaction rate. Rock on.


Name:  Adrienne & Laura B.
Username:  lbang@brynmawr.edu
Subject:  
Date:  2002-10-09 15:10:01
Message Id:  3234
Comments:

Adrienne & Laura B.

First Data Set:
   Vol. O2 in 15 sec. increments:
      .7, 1.9, 2.3, 2.6, 2.8, 2.9, 2.9, 2.9, 2.9, 2.9, 2.9

Second Data Set:
   Vol. O2 in 15 sec. increments with pH 2.0:
      Trial #1: .2, .2, .3, .4, .4, .5, .7, .9, .9, .9, .9, 1.0, 1.0, 1.0, 1.1, 1.1, 1.1, 1.1, 1.1
      Trial #2: .7, .7, .8, .9, .9, .9, .9, .9, 1.0, 1.0, 1.0, 1.0, 1.1, 1.1, 1.1, 1.1, 1.1, 1.2, 1.2

      Trial #1 Reaction Rate = .05 O2 per minute
      Trial #2 Reaction Rate = .1 O2 per minute

The Story:
According to our observations, a change in the pH of the buffer slows the reaction down. The first experiment that we did as a class with a pH 7.4 buffer had an average reaction rate of about .7 O2 per minute, while our experiment with a pH 2.0 buffer had a reaction rate of about .075 O2 per minute.


Name:  Lawral and Joanna
Username:  Anonymous
Subject:  Hydrogen Peroxide
Date:  2002-10-09 15:12:11
Message Id:  3235
Comments:
Larwal Kenrow and Annaoj Nosugref


Experiment #1:
All measurements were taken at 15 second intervals.
.8, 1, 1.2, 1.6, 2, 2.4, 2.5, 2.7, 2.8, 2.9, 3, 3, 3.1, 3.2, 3.2, 3.2
The solution reacted for 4 full minutes (240 seconds).

Experiment #4c:
3.00cc of pH 10.4 buffer was added to the original experiment.
All measurments were taken at 15 second intervals.
.6/.6, .8/.8, 1/1.2, 1.3/1.4, 1.6/1.7, 2/2, 2.2/2.3, 2.4/2.6, 2.7/2.8, 2.9/3, 3.1/3.2, 3.2/3.4, 3.4/3.5, 3.5/3.6, 3.6/3.8, 3.7/3.9, 3.8/4, 3.8/4.1, 3.9/4.2, 4/4.3, 4/4.4, 4.1/4.4, 4.2/4.4, 4.2, 4.2
The solution reacted for 375sec/345sec.

When a higher pH was added to the experiment, the solution reacted for a longer time. The rate of reaction was also higher. In the original solution, the rate of reaction was .4 O2/min. The average rate for the experiment with the added pH was .692 O2/min. The higher pH level is condusive to the reaction; it helps remove more oxygen for a longer amount of time.


Name:  
Username:  Anonymous
Subject:  Not just for hair
Date:  2002-10-09 15:16:13
Message Id:  3236
Comments:
Diana DiMuro
Erin Myers

Volume Gas reading every 15 seconds (ml)
Experiment 1: 0.2, 0.4, 0.8, 1.4, 1.6, 2.0, 2.2, 2.5, 2.7, 2.8, 2.9, 3.0, 3.1, 3.2, 3.2, 3.3, 3.4, 3.4, 3.4, 3.5, 3.6, 3.6, 3.6, 3.6
rate volume O2/min (0-2mins): 1.3
rate volume O2/min (1-3mins): 0.8
rate volume O2/min (1-4mins): 0.633


Experiment 3 increase in amount of calalase
3a: 0.6,1.4,2.0,2.4,2.8,3.1,3.2,3.4,3.5,3.6,3.7,3.8,3.8,3.8,3.9,4.0,4.0,4.1,4.2,4.2,4.2,4.2

rate volume O2/min (0-2mins): 1.7

3b:
0.4, 0.8, 1.4, 2.0, 2.4, 2.7, 2.8, 3.0, 3.0, 3.1, 3.2, 3.2, 3.4, 3.4, 3.5, 3.6, 3.6, 3.6, 3.6

rate volume O2/min (0-2mins): 1.5
rate volume O2/min (1-3mins): 0.7

3c:
0.8, 1.6, 2.2, 2.6, 3.0, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.8, 3.9, 4.0, 4.1, 4.1, 4.1, 4.2, 4.2, 4.2

rate volume O2/min (0-2mins): 1.7

An increase in the amount of calalase yielded an increase in the rate of production of O2 gas. It did not yield more O2 gas.


Name:  Diana and Will
Username:  Anonymous
Subject:  enzyme numbers
Date:  2002-10-09 15:25:03
Message Id:  3237
Comments:
Trial 1:
x (not recorded), .7, 1.1, 1.6, 2.1, 2.4, 2.7, 2.9, 3.1, 3.3, 3.4, 3.5, 3.5, 3.6, 3.7, 3.8, 3.8, 3.9, 4.0, 4.0, 4.1, 4.1, 4.2, 4.2, 4.2
total time: 3 minutes
Rater from minute 2 to minute 5: .4 O2/minute

Trial 2 (less enzyme):
.2, .3, .4, .6, .8, 1.0, 1.2, 1.4, 1.6, 1.8, 2.0, 2.2, 2.4, 2.5, 2.6, 2.8, 2.9, 3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.5, 3.6, 3.7, 3.7, 3.8, 3.9, 3.9, 4.0, 4.0, 4.0
total time: 8 minutes 15 seconds
Rate from minute 2 to minute 5: .6 O2/minute

Trial 3 (less enzyme):
.1, .2, .3, .4, .6, .75, .9, 1.1, 1.3, 1.5, 1.8, 1.9, 2.1, 2.2, 2.4, 2.6, 2.8, 2.9, 3.0, 3.1, 3.2, 3.4, 3.5, 3.6, 3.7, 3.8, 3.8, 3.9, 4.0, 4.1, 4.2, 4.2, 4.3, 4.3, 4.3
total time: 8 minutes 45 seconds
Rate from minute 2 to minute 5: .66 O2/minute

Trial 4 (less enzyme):
.0, .1, .2, .4, .5, .6, .8, 1.0, 1.1, 1.3, 1.4, 1.6, 1.8, 1.9, 2.0, 2.2, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3.0, 3.05, 3.1, 3.3, 3.4, 3.45, 3.6, 3.6, 3., 3.8, 3.9, 4.0, 4.0, 4.0
total time: 9 minutes
Rate from minute 2 to minute 6: .56 O2/minute


Name:  
Username:  stan@brynmawr.edu
Subject:  
Date:  2002-10-08 15:28:01
Message Id:  3210
Comments:
M.R., Sarah Tan

Change in catalase concentration--Experimanet #3 Trial A

For trial 1, the control, our volume of oxygem as measured every 15 seconds is as follows 0,0,0,0,.20.4,.6,.9,1.2,1.5,1.8,2.0,2.2,2.4,2.6,2.8,3.0,3.1,3.2,3.4,3.5,3.6,3.75,3.9,4.0,4.1,4.2,4.25,4.4,4.4,4.45,4.5,4.5,4.5
The reaction rate from 2 minutes to 6 minutes is 0.75 oxygen per minute.

In trial 2, we used 0.5 cc of the catalase in the experimental. Our volume of oxygen as measured every 15 seconds is as follows
0,0,0.5,0.08,0.09,0.1,0.11,0.12,0.15,.20,.22,.25,.28,.31,.34,.?,.8,.9,1.0,1.01,1.1,1.2,1.21,1.3,1.38,1.4,1.5,1.55,1.6,1.62,1.65,1.8,1.85,1.9,1.9,2.0,2.0,2.1,2.1,2.2,2.2,2.21,2.3,2.35,2.35,2.4,.2.4,2.4
The reaction rate from 2 minutes to 6 is 0.295 oxygen per minute.

In trial 3, using the same amount of catalase as trial 2, our volume of oxygen as measured every 15 seconds is as follows
0,0,.1,.1,.1,.1,.15,.2,.23,.25,.3,.4,.5,.5,.6,.65,.7,.8,.82,.9,.95,1.0,1.05,1.1,1.2,1.23,1.3,1.4,1.42,1.5,1.55,1.6,1.63,1.7,1.75,1.8,1.82,1.9,1.95,1.98,2.0,2.05,2.1,2.15,2.2,2.22,2.25,2.3,2.3,2.4,2.41,2.42,2.45,2.47,2.5,2.55,2.6,2.6,2.62,2.64,2.67,2.69,2.72.75,2.8,2.81,2.82,2.85,2.88,2.9,2.9,2.91,2.93,2.95,2.97,2.99,3.01,3.03,3.03,3.04,3.05,3.07,3.12,3.15
The reaction rate from 2 minutes to 6 is 0.225 oxygen per minute.

As you can tell, the last two trials took a longer amount of time to plateau. Since the only difference between trials 2 and 3 from trial 1 is the amount of catalase, we can conclude that this is the factor in determining the speed of a chemical reaction. We then gathered that the less amount of catalase, the slower the reaction rate.


| Biology 103 | Course Forum Area | Biology | Serendip Home |

Send us your comments at Serendip

© by Serendip 1994- - Last Modified: Wednesday, 02-May-2018 10:53:18 CDT